M45 provides a for laboratorians: follow the M45 method, and your results are defensible. Deviate, and you bear the liability.
A zoonotic pathogen with unique intrinsic resistance profiles (e.g., resistance to vancomycin).
, titled "Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria." Current Edition: clsi document m45 pdf
Note: It is highly recommended to use the latest edition to ensure adherence to current medical standards, as CLSI revises these documents regularly to incorporate new findings in antibiotic resistance.
(Note: CAMHB = Cation-Adjusted Mueller-Hinton Broth; LHB = Lysed Horse Blood) Conclusion M45 provides a for laboratorians: follow the M45
Complete Guide to CLSI M45: Infrequently Isolated or Fastidious Bacteria
| Edition | Year | Key Changes | |---------|------|--------------| | M45-A | 2005 | First edition; introduced methods for 22 organism groups. | | M45-A2 | 2010 | Added breakpoints for Burkholderia cepacia , Stenotrophomonas maltophilia . | | M45-A3 | 2015 | Expanded to include Aeromonas , Vibrio , and Plesiomonas . Updated QC strains. | | | 2020+ | Current edition. Includes new breakpoints for Campylobacter , revised Haemophilus testing, and quality control updates. | , titled "Methods for Antimicrobial Dilution and Disk
Using standard AST methods on fastidious or rare organisms can lead to inaccurate results. This can cause inappropriate patient treatment or unrecognized antibiotic resistance. Key Benefits of Implementing CLSI M45:
: "Breakpoints" (susceptible, intermediate, or resistant) tailored to the unique growth characteristics of these bacteria.